Mitotic abnormalities, chromosomal behavior, and protein content in wheat (Triticum durum) due to imazethapyr (IM) treatment were determined. Wheat seeds (variety HI-8663) were exposed to 5 IM concentrations (, , , , and 5 ppm) according to the between paper method, in laboratory conditions. | M. HOSEINY RAD, A. A. AIVAZI, S. JAGANNATH Turk J Biol 35 (2011) 663-670 © TÜBİTAK doi: Cytogenetic and biochemical effects of imazethapyr in wheat (Triticum durum) Manijeh HOSEINY RAD1, Ali Ashraf AIVAZI2, Shobha JAGANNATH3 1 Payame Noor University, Ilam Center, Dept. of Biology, Ilam - 2 Ilam University of Medical Sciences, Dept. of Public Health, Ilam - 3 University of Mysore, DOS in Botany, Mysore, 570006 - INDIA Received: Abstract: Mitotic abnormalities, chromosomal behavior, and protein content in wheat (Triticum durum) due to imazethapyr (IM) treatment were determined. Wheat seeds (variety HI-8663) were exposed to 5 IM concentrations (, , , , and 5 ppm) according to the between paper method, in laboratory conditions. The protein content in the root-shoot axis and endosperm decreased significantly with the dosage increase. Cytological observations showed that the mitotic frequency in the root meristematic cells decreased parallel to the increase in concentrations. The most common types of the observed anomalies were sticky chromosomes, lagging chromosomes, scattered chromosomes, chromatin bridges, and micronuclei. The results revealed that the herbicide IM could affect biochemical parameters, mitotic frequency, and chromosomal behavior in wheat seeds. Key words: Imazethapyr, Triticum durum, chromosomal abnormalities, mitosis, protein Introduction Food shortage is a serious global problem in the current century; thus, the agricultural sector has to increase crop grain production (1). Triticum durum, also called durum wheat or macaroni wheat, is the only tetraploid species of wheat having more commercial importance that is widely cultivated nowadays (2). Therefore, it is the most important and strategic cereal in most developing countries. Among all of the different pests, weeds are the most considerable constraint in crop production, responsible for heavy yield losses (3). Hand weeding and
