This study investigated the long-term adaptation of a rumen anaerobic fungus, Neocallimastix hurleyensis, grown on each of 3 different substrates as the sole energy source. The rumen anaerobic fungus was cultivated on Avicel, Xylan and glucose for up to 31 subcultures, and its survival and enzymatic activity were monitored. | Turk J Biol 30 (2006) 157-162 © TÜB‹TAK Effects of Sequential Sub-Culturing on the Survival and Enzyme Activity of Neocallimastix hurleyensis Mehmet Sait EK‹NC‹, Emin ÖZKÖSE, ‹smail AKYOL Department of Animal Science, Faculty of Agriculture, Kahramanmarafl Sütçü ‹mam University, 46060 Kahramanmarafl - TURKEY Received: Abstract: This study investigated the long-term adaptation of a rumen anaerobic fungus, Neocallimastix hurleyensis, grown on each of 3 different substrates as the sole energy source. The rumen anaerobic fungus was cultivated on Avicel, Xylan and glucose for up to 31 subcultures, and its survival and enzymatic activity were monitored. The fungus was able to survive on each of these substrates. Significant increases in the xylanase, avicelase and carboxymethylcellulase (CMCase) activities of N. hurleyensis were observed after 31 subcultures on Avicel and Xylan. However, there was a significant decrease in these enzyme activities after 31 consecutive subcultures of the N. hurleyensis fungus on the medium containing glucose as the sole energy source. Key Words: Anaerobic fungi, Neocallimastix hurleyensis, xylanase, avicelase, CMCase Neocallimastix hurleyensis’in Ard›fl›k Olarak Alt Kültüre Al›nmas›n›n Yaflam›na ve Enzim Aktivitesine Etkileri Özet: Bu çal›flmada rumen fungusunun farkl› üç enerji kayna¤› üzerinde yetifltirilerek uzun süreli adaptasyonu incelenmifltir. Rumen anaerobik fungusu Neocallimastix hurleyensis avisel, ksilan ve glikoz üzerinde 31 alt kültüre kadar yetifltirilmifl ve fungusun hayatta kalabilmesi ve enzimatik aktivitesi gözlemlenmifltir. Anaerobik fungusun bu enerji kaynaklar›n›n her biri üzerinde yaflayabildi¤i gözlemlenmifltir. N. hurleyensis’in ksilan üzerindeki 31’nci alt kültürden sonraki ksilanaz aktivitesinde ve avisel üzerindeki 31’nci alt kültürden sonraki aviselaz ve karboksimetilselulaz aktivitelerinde önemli miktarda art›fl oldu¤u gözlemlenmifltir. N. hurleyensis’in enerji kayna¤› olarak glikoz içeren besi .
