The effect of crude extracts, obtained from Aconitum nasutum, Daphne glomerata, Hypericum androsaemum, Laurus nobilis, Nerium oleander, Olea europaea, Prunus laurocerasus, Punica granatum, Rhododendron caucasicum, and Urtica dioica on the replication of Autographa californica nuclear polyhedrosis virus (AcNPV) grown in Spodoptera frugiperda cell culture were investigated by observing changes in cytophatic effects, progeny virus concentration and viral protein (polyhedrin) synthesis. | Turk J Biol 24 (2000) 833–844 © TÜBİTAK Antiviral Activity of Some Plant Extracts on the Replication of Autographa californica Nuclear Polyhedrosis Virus Ömer ERTÜRK, Zihni DEMİRBAĞ, Ali Osman BELDÜZ Karadeniz Teknik Üniversitesi, Fen-Edebiyat Fakültesi, Biyoloji Bölümü, Trabzon-TURKEY Received: Abstract: The effect of crude extracts, obtained from Aconitum nasutum, Daphne glomerata, Hypericum androsaemum, Laurus nobilis, Nerium oleander, Olea europaea, Prunus laurocerasus, Punica granatum, Rhododendron caucasicum, and Urtica dioica on the replication of Autographa californica nuclear polyhedrosis virus (AcNPV) grown in Spodoptera frugiperda cell culture were investigated by observing changes in cytophatic effects, progeny virus concentration and viral protein (polyhedrin) synthesis. We determined an inhibitory effect on cytophatic changes, virus concentration and synthesis of polyhedrin by utilizing the extract of A conitum nasutum, Hypericum androsaemum, Laurus nobilis, Rhododendron caucasicum, and Urtica dioica plants. On the other hand, the effects of Daphne glomerata, Nerium oleander, Olea europaea, Prunus laurocerasus and Punica granatum, extracts on the cytophatic changes and synthesis of polyhedrin were determined to be similar to the control. However, they enhance the virus concentration significantly. Key Words: Baculovirus, plant extract, antiviral, virus replication. Bazı Bitki Özütlerinin Autographa californica Nüklear Polihedrozis Virus Replikasyonuna Etkileri Özet: Bu çalışmada, Aconitum nasutum, Daphne glomerata, Hypericum androsaemum, Laurus nobilis, Nerium oleander, Olea europaea, Prunus laurocerasus, Punica granatum, Rhododendron caucasicum, ve Urtica dioica bitkilerinden elde edilen özütlerin Autographa californica nüklear polihedrozis virüs (AcNPV)'ün Spodoptera frugiperda hücre kültürlürindeki replikasyonlarına etkileri: sitopatik oluşumlar, üretilen virüs konsantrasyonu ve virüse ait polihedrin protein sentezi yönünden .
