The analysis of Rhodococcus ruber in different growth conditions using pyrolysis mass spectrometry (PyMS)

Batch grown cultures of Rhodococcus ruber from different growth phases and continuous grown cultures from carbon, phosphate and magnesium limitation were analysed by pyrolyis mass spectrometry (PyMS). | Turk J Biol 25 (2001) 333-341 © TÜB‹TAK The Analysis of Rhodococcus ruber in Different Growth Conditions Using Pyrolysis Mass Spectrometry (PyMS) Ergin KAR‹PTAfi1,2, Kamil IfiIK1,2, Nevzat fiAH‹N1,2, Reflit ÖZKANCA1, Alan C. WARD2* 1 Department of Biology, The Faculty of Science and Letters, Ondokuz May›s University, 55139, Kurupelit / Samsun - TURKEY 2 Department of Microbiology, The Medical School, University of Newcastle upon Tyne, NE2 4HH, ENGLAND Received: Abstract : Batch grown cultures of Rhodococcus ruber from different growth phases and continuous grown cultures from carbon, phosphate and magnesium limitation were analysed by pyrolyis mass spectrometry (PyMS). Batch grown cells showed significantly different pyrograms and the stationary phase cells could be clearly distinguished from exponential phase cells. Mass pyrograms of carbon, phosphate and magnesium limited cells were clearly different and, for carbon and phosphate limitation, clear trends were seen with different dilution rates. Changes in pH and temperature had no systematic effect on mass pyrograms. Key Words: Rhodococcus ruber, PyMS, Environmental conditions, Continuous culture Farkl› Ortam fiartlar›nda Yetifltirilen Rhodococcus ruber’in Pirolizis Kütle Spektrometri (PyMS) ile Analizi Özet : Kapal› kültür ortam›nda (kesikli sistem), farkl› büyüme evrelerinde yetiflen, Rhodococcus ruber’in saf hücre örnekleri ile aç›k kültür ortam›nda (kesiksiz sistem), karbon, fosfat ve magnezyum limitlenmesi yap›larak yetiflen hücre örnekleri, pirolizis kütle spektrometrisi (PyMS) ile analiz edilmifltir. Kapal› kültür ortam›nda yetiflen hücreler farkl› piyrogramlar göstermifl ve durgun fazdaki hücrelerin, logaritmik fazdaki hücrelerden aç›k bir flekilde ayr›ld›¤› belirlenmifltir. Karbon, fosfat ve magnezyum limitlenmesi yap›lan hücrelerin kütle piyrogramlar› belirgin bir flekilde farkl›d›r ve farkl› oranlardaki besi yeri ak›fl h›z›na ba¤l› olarak karbon ve fosfat limitlenmesi yap›lan .