The levels of endogenous form of free, bound and total-gibberellic acid (GA3), abscisic acid (ABA) and cytokinin (zeatin) in culture medium were determined. The changes in dry weight of the mycelium, dependent on the culture periods, was examined through the use of white-rot fungus Lentinus tigrinus and brown-rot fungus Laetiporus sulphureus which were cultured in the medium of olive oil mill waste in a static culture. | Turk J Biol 25 (2001) 453-462 © TÜB‹TAK GA3, ABA and Cytokinin Production by Lentinus tigrinus and Laetiporus sulphureus Fungi Cultured in the Medium of Olive Oil Mill Waste* Birgul ÖZCAN Mustafa Kemal University, Art and Science Faculty, Biology Department, 31040, Hatay - TURKEY S. Fatih TOPÇUO⁄LU Akdeniz University, Art and Science Faculty, Biology Department, 07058, Antalya - TURKEY Received: Abstract: The levels of endogenous form of free, bound and total-gibberellic acid (GA3), abscisic acid (ABA) and cytokinin (zeatin) in culture medium were determined. The changes in dry weight of the mycelium, dependent on the culture periods, was examined through the use of white-rot fungus Lentinus tigrinus and brown-rot fungus Laetiporus sulphureus which were cultured in the medium of olive oil mill waste in a static culture. Spectrophotometric techniques were used to determine the amounts of endogenous GA3, ABA and zeatin. As a result, fungi used in this study showed that they synthesized GA3, ABA and zeatin as a primary or secondary metabolite and these plant hormones were found to be in free and bounded forms. Key Words: Lentinus tigrinus, Laetiporus sulphureus, Fungi, Olive mill waste water, Gibberellic acid (GA3), Abscisic acid (ABA), Zeatin, Plant hormones. Zeytinya¤› Fabrikas› At›¤›nda Üretilen Lentinus tigrinus ve Laetiporus sulphureus Funguslar›ndan GA3, ABA ve Sitokinin Üretimi Özet: Statik inkübasyon ortam›nda ve zeytinya¤› fabrikas› at›¤› besiyerinde üretilen beyaz-çürükçül fungus Lentinus tigrinus ve kahverengi-çürükçül fungus Laetiporus sulphureus’un kültür ortam›nda kültür periyoduna ba¤l› olarak serbest-, ba¤l›- ve total formlarda içsel Gibberellik asit (GA3), Absisik ait (ABA) ve Sitokinin (zeatin) miktarlar› ve funguslar›n kuru misel a¤›rl›klar› saptanm›flt›r. ‹çsel GA3, ABA ve zeatin miktarlar›n›n saptanmas›nda spektrofotometrik teknik kullan›lm›flt›r. Sonuç olarak, bu çal›flmada kullan›lan funguslar›n hem serbest hemde ba¤l› formlarda
