This study reports, for the first time, an efficient in vitro plant regeneration protocol for Digitalis lamarckii Ivan. Via direct shoot organogenesis. Two sets of experiments were carried out; the first compared different concentrations of 6-benzylaminopurine (BAP), kinetin, thidiazuron (TDZ), and zeatin alone using leaf explants excised from in vitro germinated seedlings, while the second set tested the combinations of indole-3-butyric acid (IBA). | S. K. VERMA, B. B. YÜCESAN, G. ŞAHİN, S. GÜREL, E. GÜREL Research Article Turk J Bot 35 (2011) 689-695 © TÜBİTAK doi: Direct shoot regeneration from leaf explants of Digitalis lamarckii, an endemic medicinal species Sandeep Kumar VERMA1, Bahtiyar Buhara YÜCESAN1, Günce ŞAHİN1, Songül GÜREL2, Ekrem GÜREL1,* 1 Abant İzzet Baysal University, Department of Biology, 14280 Bolu - TURKEY 2 Sugar Institute, Department of Plant Breeding, 06790 Etimesgut, Ankara - TURKEY Received: Accepted: Abstract: This study reports, for the first time, an efficient in vitro plant regeneration protocol for Digitalis lamarckii Ivan. (dwarf foxglove) via direct shoot organogenesis. Two sets of experiments were carried out; the first compared different concentrations of 6-benzylaminopurine (BAP), kinetin, thidiazuron (TDZ), and zeatin alone using leaf explants excised from in vitro germinated seedlings, while the second set tested the combinations of indole-3-butyric acid (IBA) with BAP, kinetin, TDZ, and zeatin for shoot multiplication from the leaf explants, which were already cultured and developed numerous shoots during the first set of experiments. For shoot regeneration (the first set of experiments), TDZ was the most effective at mg L-1 concentration, producing a mean of shoots per explant and was significantly more effective than BAP. For shoot multiplication (the second set of experiments), a combination of mg L-1 IBA with mg L-1 TDZ produced significantly more shoots per explant ( shoots) than with BAP ( shoots), zeatin ( shoots), or kinetin ( shoots). The regenerated shoots were readily rooted on medium containing mg L-1 indole3-acetic acid (IAA). Rooted regenerants were then transferred to the pots, where they grew well and attained maturity. The described protocol provides a simple way to regenerate plants through direct shoot organogenesis, which would be useful for a large-scale production of
