Trong nghiên cứu này, TBT được xử lý cùng các cytokine TNF-α, INF-ϒ, IL-2 và IL-10, tế bào sau khi thu hoạch được xác định sự phosphoryl hóa của protein STAT-1 và STAT-3, nồng độ TNF-α trong môi trường dịch huyền phù và số lượng tế bào phản ứng dương tính với các marker Annexin V và caspase 3. | Vai trò điều hòa quá trình apoptosis của il-10 và mối liên hệ với tín hiệu stat-1 trong tế bào tua TAP CHI SINH HOC 2019, 41(1): 109–116 DOI: REGULATION OF APOPTOSIS BY IL-10 AND THE ASSOCIATION WITH STAT-1 SIGNALING MOLECULE IN DENDRITIC CELLS Nguyen Thu Thuy1, Nguyen Thi Xuan2,* 1 Institute of Biomedicine and Pharmacy, Vietnam Military Medical University Ha Noi, Vietnam 2 Institute of Genome Research, VAST, Vietnam Received 1 June 2018, accepted 1 March 2019 ABSTRACT IL-10 is an anti-inflammatory cytokine, participating in induction of immune tolerance and cell apoptotic death. Dendritic cells (DCs) is the most professional antigen-presenting cells among innate immune cells to exert generation and maintenance of immunological memory mediated through activation of T and B lymphocytes. The STAT signalling pathway plays a regulatory role of maturation and differentiation of immune cells. In this study, DCs were treated with inflammatory cytokines including TNF- , INF , IL-2 and IL-10 and subsequently examined the phosphorylation of STAT-1 and STAT-3, TNF-α concetration in cell suspension and the proportion of Annexin V+ and caspase 3+ cells. Methods used for this investigation include western blotting, flow cytometry and ELISA. DCs were derived from mouse bone marrow cells and cultured with GM-CSF for 8 days. As a result, IL-10, but not other cytokines enhanced the number of Annexin V+cells and caspase 3 activity in DCs. More importantly, IL-10 also increased the phosphorylation of STAT-1 as well as the release of TNF-α into cell suspension. In conclusion, activation of STAT-1 might relate to the cell apoptotic death and TNF-α sectetion in IL-10-treated DCs. Keywords: Apoptosis, dendritic cell, IL-10 and STAT. Citation: Nguyen Thu Thuy, Nguyen Thi Xuan, 2019. Regulation of apoptosis of dendritic cells by IL-10 and in association with STAT-1 signaling molecule. Tap
