In vitro cultures of five moss species were established on hormone-free MS medium, or on MS medium supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid and kinetin. In vitro culture of Eurhynchium praelongum (Hedw.) B., S. & G., has been initiated from the apical shoots of the gametophytes, and the cultures of Aloina aloides () Kindb. Brachythecium velutinum (Hedw.) B., S. & G., Ceratodon purpureus (Hedw.) Brid. and Grimmia pulvinata (Hedw.) Sm. were initiated from the spores of immature sporophytes. | In vitro culture of mosses: Aloina aloides () Kindb., Brachythecium velutinum (Hedw.) . & G., Ceratodon purpureus (Hedw.) Brid., Eurhynchium praelongum (Hedw.) . & G. and Grimmia pulvinata (Hedw.) Sm.