The antimicrobial activity of Azadirachta indica leaf extract (Neem leaf) was carried out on Staphylococcus aureus, Pseudomonas aeruginosa (ATCC 27853) and Escherichia coli using the agar well diffusion method. Ethanolic, aqueous and methanolic extracts of the plant were used at varying concentrations of 200 mg/ml, 100 mg/ml, 50 mg/ml, 25 mg/ml, mg/ml, mg/ml, mg/ml and mg/ml respectively. The three extracts were found to show a very high antimicrobial activity though the ethanolic extract possessed more activity than the methanolic and aqueous extracts. The minimum inhibitory concentration (MIC) was found to range from mg/ml to the three extracts respectively. When compared with standard antibiotics, the ethanolic and methanolic extracts showed maximum inhibition on these organisms. The phytochemical analyses revealed the presence of alkaloids, saponins, tannins, glycosides, steroids, terpenoids, and reducing sugars. Leaf extract of Azadirachta indica is therefore recommended for the treatment of human infections associated with these organisms. | Antimicrobial activity of azadirachta indica (Neem) leaf extract on some bacteria
