A split herd design experiment was undertaken on an organized dairy farm at Village Chimna Kalan near Jagraon, Ludhiana, Punjab (India) to test the benefit of pre-milking in addition to post-milking teat disinfection on new mastitis levels. A Lactic acid based germicidal pre-dip (‘B4-MTM’ from Hester biosciences Ltd, Ahmadabad) was applied manually using foaming cups, over a complete lactation. Post milking teat disinfection was done using Povidone iodine based germicidal post milking dip (Povidone Iodine: Glycerine 4:1). The study involved three groups with 30 cows in experimental design. Group 1 (n = 6): Cows with no teat disinfection (control); Group 2 (n = 12): Cows with post milking teat disinfection only and Group 3 (n = 12): Cows with pre and post milking teat disinfections. The quarter foremilk samples were analyzed for microbial culture, California Mastitis Test (CMT) score and somatic cell count (SCC). The CCM samples were evaluated for Electrical Conductivity (EC), pH, SCC and CMT. The prevention of new IMI was also assessed during the study period. There was a significant reduction in the values of Log10SCC in the group 2 . post milking teat disinfection while the changes in CMT, EC, and pH were non-significant. There was increase in prevalence of specific SCM from to in control group. In post milking teat disinfection there was significant reduction in specific SCM prevalence (). And in pre + post milking teat disinfection also there was significant reduction in prevalence of specific SCM vis. . However, the difference between reduction by post milking and pre + post milking teat disinfection was found to be non-significant. The prevention of new IMI was also assessed and it was observed that there was significant reduction in post milking and pre + post milking teat disinfection as compared to control group. But the difference between post and pre + post milking teat disinfection was not significant. | Comparison of post milking teat disinfection alone with pre and post milking teat disinfection in the prevention of bovine mastitis
