Biochemical variability among the 15 isolates of Alternaria isolates from different locations of West Bengal was investigated in respect of isozyme to observe the polymorphism among the isolates. Electrophoretic separation of the extracts was carried out on native PAGE following the procedure and gels were stained for different enzymes. The activity of α- esterase produced distinctive bands that were dark brown, whereas the activity of β- esterase produced bands that were dark pink so that α- esterase and βesterase were scored very easily. | Identification of biochemical variation in alternaria isolates of onion plant
