Dihydrofolate reductase (DHFR) is an important target for antimetabolite class of antimicrobials because it participates in purine synthesis. 2-mercaptobenzimidazole (2MBI) has similar structural features as purine nucleotides. | 4- 2- 1H-Benzo d imidazol-2-ylthio acetamido -N- substituted phenyl benzamides Design synthesis and biological evaluation