Two-dimensional gel electrophoresis of protein profile from groundnut (Arachis hypogaea sp. Virginia) at flower development stage

Protein is play critical role in flower development. Profiles of total flower development proteins isolated from different stages (Bud, Flower and Peg) peanut cultivar GJG-22, were studied using two-dimensional gel electrophoresis. Protein is extracted by trichloro acetic acid/acetone precipitation method. Comparative analysis was carried out of spots identified using platinum master software at three different flower stages. Protein revealed that total 9193 protein spots with the pH range 4 - 7 and 23-312 kDa range recorded. Out of 9193 spots, maximum 3413 spots were found in pH range 6 - 7 in groundnut samples. Proteomic analysis reveals the translational products of gene expression of plant during flowering stage. | Two-dimensional gel electrophoresis of protein profile from groundnut Arachis hypogaea sp. Virginia at flower development stage

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