One of the most common causes of eye infections is bacteria. The symptoms of these infections in humans can vary from mild to life-threatening complications. This study was conducted to determine the Gram negative bacterial pathogens that infect the eyes of people whether wearing lenses or not & investigate the inhibitory effect of some antibiotics and compare them with the antibacterial effect of black & green tea extracts, honey (at 10%) and CFS of Lactobacillus casei that isolated from milk of healthy mother against the pathogens isolated from the eye by using the agar diffusion technique. A total of 30 samples collected from infected eyes by swabs and transferred to the laboratory for microbial culturing. 20 different isolated species of Gram negative bacteria were identified by Vitek-2 system are (Enterobacter cloacae, Pseudomonas luteola, Leclercia adecarboxylata & pantoea agglomerans). | Lactobacillus casei isolated from human milk and three natural agents act as antibacterial against gram negative pathogenic bacteria isolated from infected eye