In vitro regeneration and in planta transformation protocols were standardized for the black gram (Vigna mungo L.) cv. VBN 3, an important grain legume. An efficient regeneration protocol was developed using 1-week-old de-embryonated cotyledon as explants. The maximum frequency of shoot regeneration (), multiple shoot induction ( ± ) and shoot elongation was achieved on MS medium supplemented with mg/l 6-benzyl amino purine (BAP) after 4 weeks of culture. Up to 65% of the regenerated shoots were rooted on MS medium containing mg/l α-naphthalene acetic acid (NAA) within 3 weeks of culture. The in vitro-raised plantlets were successfully hardened first under culture room conditions with 62% survival rate and then in greenhouse. | Studies on agrobacterium mediated in planta genetic transformation in black gram Vigna mungo L. Cultivar VBN 3
