The genetic fidelity of in vitro raised gerbera plants multiplied through micropropagation upto fifteen in vitro subcultures was assessed by using intersimple sequence repeat (ISSR) markers. 40 ISSR primers screened, 20 ISSR primers produced a total of 54 ISSRs clear, distinct and reproducible amplicons, which were monomorphic across all micropropagated plants (15) studied. Thus, a total 1094 bands were generated which exhibited homogeneous banding patterns with ISSR markers. All banding profiles from micropropagated plants were monomorphic and similar to those of the mother plant. These results indicate that the micropropagation protocol developed by us for rapid in vitro multiplication is appropriate and suitable for clonal propagation gerbera. | Assessment of genetic fidelity of micropropagated gerbera Gerbera jamesonii Bolus plants by ISSR markers