The physiological responses, dry matter and water intake of Sahiwal and Karan Fries (Holstein-Friesian X Tharparkar) heifers were monitored under two feeding regimes . feeding regime-1 (NRC, 2001) and feeding regime-2 (15 % higher energy than NRC 2001, by supplementation of molasses). Six each of healthy heifers of Sahiwal and Karan Fries (18-24 month) were selected from ICAR-National Dairy Research Institute (ICAR-NDRI), Karnal. Before start of actual experiment, the animals were given 15 days adaptation period on both the feeding regime. The physiological responses of Sahiwal and Karan Fries heifers was recorded on 1st, 7th, 14th, 15th and 16th day of each feeding regime after adaptation period. | Physiological responses and In-vitro volatile fatty acid production in cattle