Lan hài (Paphiopedilum) là một chi nhỏ với khoảng 80 loài nhưng nổi bật trong quá trình tiến hóa của họ Lan. Nghiên cứu trình bày kết quả phân tích hình thái thực vật của lan hài Vệ nữ thu thập tại Thái Nguyên, kết hợp với xác định trình tự gen trnH-psbA để nhận diện, phân nhóm loài lan này. | TNU Journal of Science and Technology 226 14 153 - 160 MORPHOLOGICAL CHARACTERISTICS AND DNA trnH-psbA GENE SEQUENCE IN THE TAXONOMY OF P. hirsutissimum Nguyen Thi Hai Yen1 Ngo Xuan Quang2 4 Nguyen Dinh Trong3 Do Tien Phat3 4 1TNU - University of Sciences 2VAST - Institute of Tropical Biology 3VAST - Institute of Biotechnology 4VAST - Graduate University of Sciences and Technology ARTICLE INFO ABSTRACT Received 08 9 2021 The Paphiopedilum is a small genus with about 80 species but stands out in the evolution of the family Orchidaceae. This genus is also quite special Revised 19 10 2021 because of the complex taxonomy system with many subgenera Published 25 10 2021 subdivisions and different taxonomic views. The advent of DNA barcodes has greatly supported taxonomic studies beyond traditional morphology- KEYWORDS based methods. This paper presents the results of morphological analysis of plant materials of the Venu orchid species collected in Thai Nguyen Taxonomy province combined with the trnH-psbA gene sequences to identify to trnH-psbA species. The results found that the trnH-psbA gene sequence from the researched Venu orchid species samples obtained a gene fragment of 612 P. hirsutissimum bp in size nucleotide similarity reached compared with the trnH- Barcode psbA sequence of species P. hirsutissimum code. on the Thai Nguyen Genbank. The taxonomic tree was established based on the trnH-psbA sequence of Venu orchid species with 19 sequences on the NCBI Genbank showing that trnH-psbA sequence is a potential indicator in the identification and classification of The Paphiopedilum. The branches on the taxonomic tree clearly reflect the relationship of these species which are grouped to subgenera subdivision much better than that in comparison with previous morphological taxonomy. ĐẶC ĐIỂM HÌNH THÁI VÀ TRÌNH TỰ GEN tnrH-psbA TRONG PHÂN LOẠI LAN HÀI VỆ NỮ P. hirsutissimum Nguyễn Thị Hải Yến1 Ngô Xuân Quảng2 4 Nguyễn Đình Trọng3 Đỗ Tiến Phát3 4 .
