The significant alterations were observed in SOD, CAT, GST and AChE activity for both oral and contact exposure pathways. In conclusion, cypermethrin modulated the activities of biomarkers of oxidative stress and altered the level of energy metabolites in the bees. | Environmental Advances 5 2021 100074 Contents lists available at ScienceDirect Environmental Advances journal homepage journal environmental-advances Effects of cypermethrin as a model chemical on life cycle and biochemical responses of the tropical stingless bee Meliponula bocandei Spinola 1853 Glory U. Chibee a Olajumoke M. Ojelabi b Hamzat O. Fajana c d Bolajoko A. Akinpelu b Temitope O. Kehinde a Olufemi M. Awodiran a Efere M. Obuotor b Olugbenga J. Owojori a a Department of Zoology Obafemi Awolowo University Ile-Ife Nigeria b Department of Biochemistry and Molecular Biology Obafemi Awolowo University Ile-Ife Nigeria c Toxicology Centre University of Saskatchewan Saskatoon SK S7N 5B3 Canada d Department of Soil Science University of Saskatchewan Saskatoon SK S7N 5A8 Canada A R T I C L E I N F O A B S T R A C T Keywords The tropical stingless bee Meliponula bocandei Spinola 1853 is an important pollinator in West Africa but there is West Africa no study on the effect of pesticides on this species. This study assessed the effects of cypermethrin a common Stingless bees pyrethroid on survival taste sucrose sensitivity and biochemical responses of M. bocandei. The biochemical Pesticides markers were superoxide dismutase SOD catalase CAT glutathione S-transferase GST reduced glutathione Ecotoxicology GSH and acetylcholinesterase AChE as well as glucose trehalose and total protein concentrations. Test temperature was optimized by acclimatizing adult worker bees collected from a pristine natural colony to different temperature regimes in the laboratory and fed with sucrose solution. The optimized temperature 22 C for survival and sucrose consumption was adopted for the toxicity test. The 24 48 h oral lethal dose LD50 and 24 96 h indirect contact lethal concentration LC50 of cypermethrin on the bees was in the range of µg ml and µg ml respectively. Also the overall PER response reduced below 50 in bees that were orally .
