This study, a range of temperatures, lighting periods and salinity levels were examined for cyst hatching. The quality nauplii from optimized cyst hatching conditions were used to determine the toxicity of 26 methanol extracts of 24 poisonous plant species collected from the mountainous regions in Quang Tri province of Vietnam. | ACADEMIA JOURNAL OF BIOLOGY 2024 46 1 55 67 DOI 2615-9023 18899 OPTIMIZATION OF BRINE SHRIMP LETHALITY TEST FOR IN VIVO TOXICITY EVALUATION OF POISONOUS PLANT SPECIES COLLECTED FROM QUANG TRI PROVINCE Nguyen Chi Mai1 2 Nguyen Tuong Van3 Pham Thi Hoe1 Vu Huong Giang1 Ninh Khac Ban1 2 Tran My Linh1 2 1 Institute of Marine Biochemistry Vietnam Academy of Science and Technology 18 Hoang Quoc Viet Ha Noi Vietnam 2 Graduate University of Science and Technology Vietnam Academy of Science and Technology 18 Hoang Quoc Viet Ha Noi Vietnam 3 VNTEST Institute for Quality Testing and Inspection - Vietnam Union of Science and Technology Associations Vietnam Received 14 September 2023 accepted 18 March 2024 ABSTRACT Plants are natural resources providing several important bioactive compounds for human health. To discover such valuable properties researchers need to focus on both the pharmacology and toxicity of plant materials. Preliminary toxicity assessment of plants using the Brine Shrimp Lethality Test is a convenient simple and effective tool. However some environmental parameters such as light temperature and salinity need to be optimized for our own laboratory conditions and Artemia salina cysts produced in Vietnam. The obtained results indicated that the continuous lighting regime temperature of 30 oC and salinity of 30 ppt are the most suitable parameters for cyst hatching within 24 hours and development of nauplii after 24 hours of hatching. Based on the optimal cyst-hatching procedure the potential toxicity of 26 extracts from 24 poisonous plant species collected in Quang Tri was determined with median lethal concentration LC50 . The results showed that 3 26 extracts were extremely toxic with LC50 10 µg mL 10 26 extracts were highly toxic with LC50 from 10 µg mL to 100 µg mL 8 26 extracts were moderately toxic with LC50 from 100 µg mL to 250 µg mL 4 26 extracts had low toxicity with LC50 from 250 µg mL to 1 000 µg mL and 1 26 extract was not toxic with LC50 .
