Handbook of Microbiological Media, Fourth Edition part 135 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Oxidation-Fermentation Medium King s 1335 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically add of sterile antibiotic inhibitor. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the isolation and cultivation of Listeria monocytogenes from specimens containing a mixed bacterial flora. Oxford Medium BAM M118 Composition per liter Special Ferric ammonium g Antibiotic pH at 25 C Source This medium is available as a premixed powder from Oxoid Unipath. Caution Lithium chloride is harmful. Avoid bodily contact and inhalation of vapors. On contact with skin wash with plenty of water immediately. Supplement Mix Composition per Colistin Ethanol 50 solution . Preparation of Supplement Mix Add components to of ethanol. Mix thoroughly. Filter sterilize. Caution Cycloheximide is toxic. Avoid skin contact or aerosol formation and inhalation. Preparation of Medium Add components except supplement mix to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 C. Aseptically add of sterile supplement mix. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the isolation and cultivation of Listeria monocytogenes from specimens containing a mixed bacterial flora. Oxidation-Fermentation Medium OF Medium Composition per liter Pancreatic digest of Bromthymol Carbohydrate pH at 25 C Source This medium is available as a premixed powder from BD Diagnostic Systems. Carbohydrate Solution Composition per Preparation of Carbohydrate Solution Add carbohydrate to distilled deionized
