Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp Original article đề tài: An improved micropropagation protocol for stone pine (Pinus pinea L.). | Ann. For. Sci. 63 2006 879-885 INRA EDP Sciences 2006 DOI forest 2006071 879 Original article An improved micropropagation protocol for stone pine Pinus pinea L. Pablo ALONSOa Paloma MONCALEÁNb Belén FERNÁNDEZa Ana RODRÍGUEZa Maria Luz CENTENOc Ricardo J. OrdaSa a Area de Fisiologia Vegetal Instituto Universitario de Biotecnología Departamento de Biología de Organismos y Sistemas Universidad de Oviedo C Catedrático Rodrigo Uria s n 330071 Oviedo Spain b Current adress Instituto Vasco de Investigation y Desarrollo Agrario Centro Arkaute Aptdo. 46 01080 Vitoria-Gasteiz Pais Vasco Spain c Departamento de Biologia Vegetal Universidad de León Facultad de Ciencias Biológicas y Ambientales Campus de Vegazana León Spain Received 9 November 2005 accepted 30 May 2006 Abstract - A protocol for micropropagation from isolated cotyledons from Pinus pinea L. has been developed. Major improvements are the increase in rooting rates and the description for the first time of a successful continuous multiplication procedure. For bud induction isolated cotyledons were cultured on 1 2 LP with pM BA during 4 days. Shoot development was obtained by transfer to 1 2 LP hormone-free medium with activated charcoal. Since an additional 20 weeks were required for shoot elongation and rooting at least 70 plantlets can be expected per seed after 29 weeks based on a 70 success rate. Besides shoots can be micropropagated successively by subculturing on 1 2 LPC. conifers micropropagation organogenesis rooting tissue culture Résumé - Amelioration du protocole de multiplication par micropropagation du pin pignon Pinus pinea L. . Nous avons développé un protocole de multiplication par micropropagation de Pinus pinea L. à partir de cotylédons isolés. Les principaux progrès réalisés concernent l augmentation du taux d enracinement et la description pour la première fois d une méthode de multiplication en continu. Pour l induction des bourgeons des cotylédons isolés ont été cultivés dans le .
