Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp Original article đề tài: Efficient method of micropropagation and in vitro rooting of teak (Tectona grandis L.) focusing on large-scale industrial plantations. | Ann. For. Sci. 64 2007 73-78 INRA EDP Sciences 2007 DOI forest 2006090 73 Original article Efficient method of micropropagation and in vitro rooting of teak Tectona grandis L. focusing on large-scale industrial plantations Emilio Mendoza de GYVESa Juwartina Ida ROYANla b Eddo RUGlNla a Dipartimento Produzione Vegetale Università degli Studi della Tuscia Via S. C. de Lellis 1 Viterbo 01100 Italy b Present address Biotech Center Agency for The Assessment and Application of Technology Kawasan Puspiptek 630 Bld Serpong Indonesia Received 12 December 2005 accepted 2 March 2006 Abstract - Multiple shoots of high quality were produced in vitro from nodal explants of Tectona grandis. An average of about 4 shoots uninodal explant was obtained within 4 weeks of culture on Murashige and Skoog s mMS medium modified by 50 reduction in NH4NO3 concentration supplemented with benzylaminopurine mg L-1 indole-3-butyric acid mg L-1 and gibberellic acid mg L-1 . The latter was applied both in the medium and by soaking the nodal segments for 10 s. in a gibberellic acid solution of 100 mg L-1. Hundred percent of shoots rooted cultured on modified MS medium containing IBA mg L-1 and putrescine 160 mg L-1 . Putrescine promoted both strong and highly ramified roots and fast growing shoots during the rooting phase conditioning the plantlets for a good survival and quality. Plantlets were transferred to jiffy pots for a short acclimatization stage in greenhouse where they survived at 100 . This highly reproducible procedure can be adopted for large scale teak propagation. plant growth regulator putrescine rooting nodal explant gibberellic acid Résumé - Développement d une méthode efficace de micropropagation et d enracinement in vitro du teak Tectona grandis L. pour une production industrielle de plants. Des pousses multiples de haute qualité ont été produites in vitro à partir d explants nodaux de Tectona grandis. Une moyenne d environ 4 pousses par explant uninodal
