Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Radioprotective effects of an acidic polysaccharide of Panax ginseng on bone marrow cells | J. Vet. Set. 2007 8 1 39-44 JOURNAL OF Veterinary Science Radioprotective effects of an acidic polysaccharide of Panax ginseng on bone marrow cells Hyun-Ji Kim1 Mi-Hyoung Kim1 Yun-Young Byon2 Jae Woo Park3 Youngheun Jee1 Hong-Gu Joo1 1 Department of Veterinary Medicine College of Applied Life Sciences 2Applied Radiological Science Research Institute iDepartment of Nuclear and Energy Engineering College of Engineering Cheju National University Jeju 690-756 Korea An acidic polysaccharide of Panax ginseng APG so called ginsan is known to have important immunomodulatory activities. It was recently reported that APG has radioprotective effects in mice but the detailed mechanism was not fully elucidated. This study examined the effects of APG on bone marrow cells BMs . The phenotypical and functional changes in APG-treated BMs after gamma radiation were studied. The benefit of APG on BMs damaged by gamma radiation was determined by measuring the cell viability. Using 2 different assays a pretreatment with APG significantly increased the viability of BMs against gamma radiation. APG-treated BMs had a significantly higher amount of IL-12 which is a major cytokine for immune responses compared with the medium-treated BMs. The expression of MHC class II molecules of APG-treated BMs was also increased and APG-treated BMs showed significantly higher levels of allogeneic CD4 T lymphocyte proliferation. Furthermore APG-treated mice had a larger number of BMs after gamma radiation than the control mice and the BMs of APG-treated mice were successfully cultured into dendritic cells which are the representative antigenpresenting cells. Overall this study shows that APG alters the phenotype of BMs increases the viability and alloreactivity of BMs after gamma radiation both in vitro and in vivo. Therefore APG may be a good candidate radioprotective agent for BMs. Key words bone marrow cells gamma radiation ginsan Panax ginseng radioprotection Introduction Panax ginseng is a .
