Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Enhancement of protective immune responses by oral vaccination with Saccharomyces cerevisiae expressing recombinant Actinobacillus pleuropneumoniae ApxIA or ApxIIA in mice | J. Vet. Sci. 2007 8 4 383-392 JOURNAL OF Veterinary Science Enhancement of protective immune responses by oral vaccination with Saccharomyces cerevisiae expressing recombinant Actinobacillus pleuropneumoniae ApxIA or ApxIIA in mice 1 f 1 1 1 2 2 Sung Jae Shin Seung Won Shin Mi Lan Kang Deog Yong Lee Moon-Sik Yang Yong-Suk Jang Han Sang Yoo1 Department of Infectious Diseases College of Veterinary Medicine BK21 for Veterinary Science and KRF Zoonotic Disease Priority Research Institute Seoul National University Seoul 151-742 Korea 2Division of Biological Science Institute for Molecular Biology and Genetics Chonbuk National University Jeonju 561-756 Korea We previously induced protective immune response by oral immunization with yeast expressing the ApxIIA antigen. The Apxl antigen is also an important factor in the protection against Actinobacillus pleuropneumoniae serotype 5 infection therefore the protective immunity in mice following oral immunization with Saccharomyces cer-evisiae expressing either ApxIA group C or ApxIIA group D alone or both group E was compared with that in two control groups group A and B . The immunogenicity of the rApxIA antigen derived from the yeast was confirmed by a high survival rate and an ApxlA-specific IgG antibody response p . The highest systemic IgG and local IgA humoral immune responses to ApxIA and ApxIIA were detected in group E after the third immunization p . The levels of IL-tp and IL-6 after challenge with an A. pleuropneumoniae field isolate did not change significantly in the vaccinated groups. The level of TNF-a increased in a time-dependent manner in group E but was not significantly different after the challenge. After the challenge the mice in group E had a significantly lower infectious burden and a higher level of protection than the mice in the other groups p . The survival rate in each group was closely correlated to the immune response and histopathological observations in the lung following the .
