Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Laparoscopy vs. laparotomy for embryo transfer to produce transgenic goats (Capra hircus) | J. Vet. Sci. 2008 9 1 103-107 JOURNAL OF Veterinary Science Laparoscopy vs. laparotomy for embryo transfer to produce transgenic goats Capra hircus 1 01 1 1x711 if Sang Tae Shin Sung Keun Jang Hong Suk Yang Ok Keun Lee Yhong Hee Shim Won Il Choi 1 2 1x71 Doo Soo Lee Gwan Sun Lee Jong Ki Cho Young Won Lee College of Veterinary Medicine Chungnam National University Daejeon 305-764 Korea 2Hanmi Research Center Hwaseong 445-813 Korea This study was performed to produce transgenic Korean native goat Capra hircus by laparoscopic embryo transfer ET to overcome the limitations of ET performed by laparotomy. Transgenic embryos were produced by DNA pronuclear microinjection of in vivo zygotes. The recipient goats were synchronized for estrus by using an introvaginal progesterone devices as a controlled internal drugreleasing insert CIDR for 13 days and injection of 400 IU PMSG 48 h before removal of the insert. Embryos were transferred on day 3 and 4 after removal of the insert. Recipient goats were deprived of feed for 48 h then suspended in a laparotomy cradle at an angle of 45o. After obtaining a sufficient pneumoperitoneum the laparoscope and forceps were inserted abdominally through 5 mm trocar sleeves. Examination of the ovaries and uterus was performed and then 213 embryos were transferred into the oviducts via the infundibula of 76 recipient goats. To compare pregnancy rates ET was also performed by laparotomy in 82 recipient goats. The pregnancies in the recipient goats were diagnosed by ultrasound on day 30 after embryo transfer. The pregnancy rate with laparoscopic ET was significantly higher than with ET performed by laparotomy vs. p . In addition the pregnancy rates were compared between ovulated and non-ovulated ovaries of the recipient goats in the laparoscopic ET group. No significant difference was observed between the pregnancy rates of ovulated and non-ovulated ovaries vs. p suggesting that ET may also be possible in .
