Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Strong inhibition of TNF-α production and inhibition of IL-8 and COX-2 mRNA expression in monocyte-derived macrophages by RWJ 67657, a p38 mitogen-activated protein kinase (MAPK) inhibitor. | Available online http content 6 4 R384 Research article Strong inhibition of TNF-a production and inhibition of IL-8 and COX-2 mRNA expression in monocyte-derived macrophages by RWJ 67657 a p38 mitogen-activated protein kinase MAPK inhibitor Johanna Westra1 Berber Doornbos-van der Meer1 Peter de Boer2 Miek A van Leeuwen1 Martin H van Rijswijk1 and Pieter C Limburg1 3 Department of Rheumatology University Hospital Groningen Groningen The Netherlands 2Pharmaceutical Research and Development Johnson and Johnson High Wycombe UK department of Pathology and Laboratory Medicine University Hospital Groningen Groningen The Netherlands Corresponding author Johanna Westra Received 18 Mar 2004 Revisions requested 8 Apr 2004 Revisions received 19 Apr 2004 Accepted 7 May 2004 Published 21 Jun 2004 Arthritis Res Ther 2004 6 R384-R392 DOI ar1 204 2004 Westra et al. licensee BioMed Central Ltd. This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Open Access Abstract In inflammatory processes the p38 mitogen-activated protein kinase MAPK signal transduction route regulates production and expression of cytokines and other inflammatory mediators. Tumor necrosis factor a TNF-a is a pivotal cytokine in rheumatoid arthritis and its production in macrophages is under control of the p38 MAPK route. Inhibition of the p38 MAPK route may inhibit production not only of TNF-a but also of other inflammatory mediators produced by macrophages and indirectly of inflammatory mediators by other cells induced by TNF-a stimulation. Here we investigate the effects of RWJ 67657 a p38 MAPK inhibitor on mRNA expression and protein production of TNF-a and other inflammatory mediators in monocyte-derived macrophages. A strong inhibition of TNF-a was seen at pharmacologically relevant concentrations of RWJ 67657 .
