Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Identification of subpopulations with characteristics of mesenchymal progenitor cells from human osteoarthritic cartilage using triple staining for cell surface markers. | Available online http content 6 5 R422 Research article Identification of subpopulations with characteristics of mesenchymal progenitor cells from human osteoarthritic cartilage using triple staining for cell surface markers Stefan Fickert1 2 Jorg Fiedler1 3 and Rolf E Brenner1 3 Open Access Department of Orthopaedics University of Ulm Ulm Germany 2Department of Orthopaedics University of Dresden Dresden Germany 3Division for Biochemistry of Joint and Connective Tissue Diseases University of Ulm Ulm Germany Corresponding author Rolf E Brenner Received 16 Apr 2004 Revisions requested 19 May 2004 Revisions received 28 May 2004 Accepted 14 Jun 2004 Published 19 Jul 2004 Arthritis Res Ther 2004 6 R422-R432 DOI ar1210 2004 Fickert et al. licensee BioMed Central Ltd. This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract We first identified and isolated cellular subpopulations with characteristics of mesenchymal progenitor cells MPCs in osteoarthritic cartilage using fluorescence-activated cell sorting FACS . Cells from osteoarthritic cartilage were enzymatically isolated and analyzed directly or after culture expansion over several passages by FACS using various combinations of surface markers that have been identified on human MPCs CD9 CD44 CD54 CD90 CD166 . Culture expanded cells combined and the subpopulation derived from initially sorted CD9 CD90 CD166 cells were tested for their osteogenic adipogenic and chondrogenic potential using established differentiation protocols. The differentiation was analyzed by immunohistochemistry and by RT-PCR for the expression of lineage related marker genes. Using FACS analysis we found Keywords cartilage mesenchymal progenitor cell osteoarthritis that various triple combinations of CD9 CD44 CD54 CD90 and CD166 positive
