Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Tumour necrosis factor-α stimulates dehydroepiandrosterone metabolism in human fibroblast-like synoviocytes: a role for nuclear factor-κB and activator protein-1 in the regulation of expression of cytochrome p450 enzyme 7b. | Available online http content 7 6 R1271 Research article Tumour necrosis factor-a stimulates dehydroepiandrosterone metabolism in human fibroblast-like synoviocytes a role for nuclear factor-KB and activator protein-1 in the regulation of expression of cytochrome p450 enzyme 7b John Dulos1 Allard Kaptein1 Annemieke Kavelaars2 Cobi Heijnen2 and Annemieke Boots1 Open Access Department of Pharmacology Section Autoimmunity . Organon Oss The Netherlands 2Laboratory for Psychoneuroimmunology University Medical Center Utrecht Utrecht The Netherlands Corresponding author John Dulos Received 17 May 2005 Revisions requested 27 Jun 2005 Revisions received 4 Aug 2005 Accepted 11 Aug 2005 Published 15 Sep 2005 Arthritis Research Therapy 2005 7 R1271-R1 280 DOI ar1819 This article is online at http content 7 6 R1 271 2005 Dulos et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Glucocorticoids have successfully been used in the treatment of rheumatoid arthritis. Data suggest that 7a-hydroxy-dehydroepiandrosterone 7a-OH-DHEA an immunostimulating metabolite of dehydroepiandrosterone can block glucocorticoid-induced immune suppression. Formation of 7a-OH-DHEA is catalyzed by activity of cytochrome p450 enzyme 7b Cyp7b . Recently we reported that tumour necrosis factor TNF -a IL-1a IL-1P and IL-17 enhance Cyp7b mRNA expression and induce a concomitant increase in the formation of 7a-OH-DHEA by fibroblast-like synoviocytes FLS from rheumatoid arthritis patients. The aim of this study was to elucidate which signal transduction pathway is involved in the TNF-a-mediated induction of Cyp7b activity in FLS. We studied the effects of inhibitors of different
