Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Expression, regulation and clinical significance of soluble and membrane CD14 receptors in pediatric inflammatory lung diseases. | Marcos et al. Respiratory Research 2010 11 32 http content 11 1 32 RESPIRATORY RESEARCH RESEARCH Open Access Expression regulation and clinical significance of soluble and membrane CD14 receptors in pediatric inflammatory lung diseases Veronica Marcos1 Phillip Latzin2 Andreas Hector1 Sebastian Sonanini4 Florian Hoffmann1 Martin Lacher1 Barbara Koller3 Philip Bufler1 Thomas Nicolai1 Dominik Hartl1 Matthias Griese1 Abstract Background Inflammatory lung diseases are a major morbidity factor in children. Therefore novel strategies for early detection of inflammatory lung diseases are of high interest. Bacterial lipopolysaccharide LPS is recognized via Toll-like receptors and CD14. CD14 exists as a soluble sCD14 and membrane-associated mCD14 protein present on the surface of leukocytes. Previous studies suggest sCD14 as potential marker for inflammatory diseases but their potential role in pediatric lung diseases remained elusive. Therefore we examined the expression regulation and significance ofsCD14 and mCD14 in pediatric lung diseases. Methods sCD14 levels were quantified in serum and bronchoalveolar lavage fluid BALF of children with infective pneumonia cystic fibrosis CF and non-infective asthma inflammatory lung diseases and healthy control subjects by ELISA. Membrane CD14 expression levels on monocytes in peripheral blood and on alveolar macrophages in BALF were quantified by flow cytometry. In vitro studies were performed to investigate which factors regulate sCD14 release and mCD14 expression. Results sCD14 serum levels were specifically increased in serum of children with pneumonia compared to CF asthma and control subjects. In vitro CpG induced the release of sCD14 levels in a protease-independent manner whereas LPS-mediated mCD14 shedding was prevented by serine protease inhibition. Conclusions This study demonstrates for the first time the expression regulation and clinical significance of soluble and membrane CD14 receptors in .
