Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:" Beta-estradiol attenuates hypoxic pulmonary hypertension by stabilizing the expression of p27kip1 in rats. | Xu et al. Respiratory Research 2010 11 182 http content 11 1 182 RESPIRATORY RESEARCH RESEARCH Open Access Beta-estradiol attenuates hypoxic pulmonary hypertension by stabilizing the expression of p27kip1 in rats l I u . I I n rs V I 1 2t I I iz S2t l I II I2t 11 yw z ì A Z 1 2 D z-s ĩ n Z 12 I I V I2 Z-i A I z i2 I_I I _ i z i rx Z-K Z 1 2 Dun-Quan Ad Ying Luo Yi Liu Jing Wang Bo Zhang Min Xu van-Xia Wang Hai-Ying Dong 2 2 2 2 z- 3 Ming-Qing Dong Peng-Tao Zhao Wen Niu Man-Ling Liu Yu-Qi Gao Zhi-Chao Li Abstract Background Pulmonary vascular structure remodeling PVSR is a hallmark of pulmonary hypertension. P27kip1 one of critical cyclin-dependent kinase inhibitors has been shown to mediate anti-proliferation effects on various vascular cells. Beta-estradiol P-E2 has numerous biological protective effects including attenuation of hypoxic pulmonary hypertension HPH . In the present study we employed p-E2 to investigate the roles of p27kip1 and its closely-related kinase Skp-2 in the progression of PVSR and HPH. Methods Sprague-Dawley rats treated with or without b-E2 were challenged by intermittent chronic hypoxia exposure for 4 weeks to establish hypoxic pulmonary hypertension models which resemble moderate severity of hypoxia-induced PH in humans. Subsequently hemodynamic and pulmonary pathomorphology data were gathered. Additionally pulmonary artery smooth muscle cells PASMCs were cultured to determine the antiproliferation effect of b-E2 under hypoxia exposure. Western blotting or reverse transcriptional polymerase chain reaction RT-PCR were adopted to test p27kip1 Skp-2 and Akt-P changes in rat lung tissue and cultured PASMCs. Results Chronic hypoxia significantly increased right ventricular systolic pressures RVSP weight of right ventricle left ventricle plus septum RV LV S ratio medial width of pulmonary arterioles accompanied with decreased expression of p27kip1 in rats. Whereas b-E2 treatment repressed the elevation of RVSP RV .