Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Characterization of a panel of six β2-adrenergic receptor antibodies by indirect immunofluorescence microscopy. | Respiratory Research BioMed Central Research Characterization of a panel of six p2-adrenergic receptor antibodies by indirect immunofluorescence microscopy Yulia A Koryakina1 2 3 Tristan W Fowler2 3 Stacie M Jones1 2 3 Bradley J Schnackenberg2 3 Lawrence E Cornett1 and Richard C Kurten 1 2 3 Open Access Address Department of Physiology and Biophysics University of Arkansas for Medical Sciences Little Rock AR 72205 USA 2Department of Pediatrics University of Arkansas for Medical Sciences AR USA and 3Arkansas Children s Hospital Research Institute Little Rock AR 72202 USA Email Yulia A Koryakina - YAKoriakina@ Tristan W Fowler - FowlerTristanW@ Stacie M Jones - JonesStacieM@ Bradley J Schnackenberg - SchnackenbergBradley@ Lawrence E Cornett - CornettLawrenceE@ Richard C Kurten - KurtenRichardC@ Corresponding author Published 18 April 2008 Respiratory Research 2008 9 32 doi 1465-9921 -9-32 Received 2 November 2007 Accepted 18 April 2008 This article is available from http content 9 1 32 2008 Koryakina et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The P2-adrenergic receptor P2AR is a primary target for medications used to treat asthma. Due to the low abundance of P2AR very few studies have reported its localization in tissues. However the intracellular location of P2AR in lung tissue especially in airway smooth muscle cells is very likely to have a significant impact on how the airways respond to P-agonist medications. Thus a method for visualizing P2AR in tissues would be of utility. The purpose of this study was to develop an immunofluorescent labeling technique for localizing native and recombinant P2AR in primary .
