Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Notochordal conditioned media from tissue increases proteoglycan accumulation and promotes a healthy nucleus pulposus phenotype in human mesenchymal stem cells. | Purmessur et al. Arthritis Research Therapy 2011 13 R81 http content 13 3 R81 RESEARCH ARTICLE Open Access Notochordal conditioned media from tissue increases proteoglycan accumulation and promotes a healthy nucleus pulposus phenotype in human mesenchymal stem cells 12 2 3 2 Devina Purmessur Rachel M Schek Rosalyn D Abbott Bryan A Ballif Karolyn E Godburn and James C Iatridis1 Abstract Introduction Notochordal cells NCs are influential in development of the intervertebral disc IVD and species that retain NCs do not degenerate. IVD repair using bone marrow derived mesenchymal stem cells MSCs is an attractive approach and the harsh microenvironment of the IVD suggests pre-differentiation is a necessary first step. The goal of this study was to use soluble factors from NCs in alginate and NCs in their native tissue to differentiate human MSCs to a young nucleus pulposus NP phenotype. Methods Human MSCs cultured under micromass conditions for 21 days in hypoxia were differentiated with conditioned medium derived from porcine notochordal cells in native tissue NCT or in alginate beads NCA and compared with chondrogenic TGFp-3 or basal medium. A PCR array of 42 genes was utilized to screen a large number of genes known to be associated with the healthy NP phenotype and pellet cultures were also evaluated for glycosaminoglycan content histology and viability. Proteomic analysis was used to assess candidate soluble factors in NCA and NCT. Results Notochordal cell conditioned media had diverse effects on MSC phenotype. NCT resulted in the highest levels of glycosaminoglycan GAG as well as up-regulation of SOX9 and Collagen II gene expression. NCA demonstrated effects that were catabolic yet also anti-fibrotic and minimally hypertrophic with down-regulation of Collagens I and III and low levels of Collagen X respectively. Micromass culture and hypoxic conditions were sufficient to promote chondrogenesis demonstrating that both basal and chondrogenic .
