Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:IL-4 induced MUC4 enhancement in respiratory epithelial cells in vitro is mediated through JAK-3 selective signaling Gautam Damera1, Baoyun Xia1 and Goverdhan P. | Respiratory Research BioMed Central Research Open Access IL-4 induced MUC4 enhancement in respiratory epithelial cells in vitro is mediated through JAK-3 selective signaling Gautam Damera1 Baoyun Xia1 and Goverdhan P Sachdev 1 2 Address 1College of Pharmacy University of Oklahoma Health Sciences Center Oklahoma City OK - 73190 USA and 2The Oklahoma Center for Medical Glycobiology University of Oklahoma Health Sciences Center Oklahoma City OK - 73104 USA Email Gautam Damera - gautam-damera@ Baoyun Xia - baoyun-xia@ Goverdhan P Sachdev - gordon-sachdev@ Corresponding author Published 21 March 2006 Received 19 November 2005 Respiratory Research2006 7 39 doi l465-992l -7-39 Accepted 2l March 2006 This article is available from http content 7 l 39 2006Damera et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Recent studies have identified MUC4 mucin as a ligand for activation of ErbB2 a receptor tyrosine kinase that modulates epithelial cell proliferation following epithelial damage in airways of asthmatics. In this study we investigated the potential role of IL-4 one of the Th2 inflammatory cytokines persistent in asthmatic airways in regulating MUC4 expression using a cell line NCI-H650 Methods Real time PCR analysis was performed to determine concentration and time dependent effects of IL-4 upon MUC4 expression. Nuclear run on experiments were carried out to explore potential transcriptional modulation. Western blotting experiments using a monoclonal antibody specific to ASGP-2 domain of MUC4 were performed to analyze MUC4 glycoprotein levels in plasma membrane fractions. To analyze potential signal transduction cascades IL-4 treated confluent
