Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: "RhoA signaling modulates cyclin D1 expression in human lung fibroblasts; implications for idiopathic pulmonary fibrosis. | Respiratory Research BioMed Central Research Open Access RhoA signaling modulates cyclin D1 expression in human lung fibroblasts implications for idiopathic pulmonary fibrosis KL Watts E Cottrell PR Hoban and MA Spiteri Address Lung Research Institute of Science and Technology in Medicine University Hospital of North Staffordshire Keele University Staffordshire UK Email KL Watts - keira_watts@ E Cottrell - elizabethcottrell@ PR Hoban - MA Spiteri - Corresponding author Published 15 June 2006 Received 13 February 2006 Respiratory Research 2006 7 88 doi l465-992l-7-88 Accepted I5 June 2006 This article is available from http content 7 l 88 2006 Watts et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Idiopathic Pulmonary Fibrosis IPF is a debilitating disease characterized by exaggerated extracellular matrix deposition and aggressive lung structural remodeling. Disease pathogenesis is driven by fibroblastic foci formation consequent on growth factor overexpression and myofibroblast proliferation. We have previously shown that both CTGF overexpression and myofibroblast formation in IPF cell lines are dependent on RhoA signaling. As RhoA-mediated regulation is also involved in cell cycle progression we hypothesise that this pathway is key to lung fibroblast turnover through modulation of cyclin Dl kinetic expression. Methods Cyclin Dl expression was compared in primary IPF patient-derived fibroblasts and equivalent normal control cells. Quantitative real time PCR was employed to examine relative expression levels of cyclin Dl mRNA protein expression was confirmed by western blotting. Effects of Rho .
