Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Anti-inflammatory activity of monogalactosyldiacylglycerol in human articular cartilage in vitro: activation of an antiinflammatory cyclooxygenase-2 (COX-2) pathway. | Ulivi et al. Arthritis Research Therapy 2011 13 R92 http content 13 3 R92 RESEARCH ARTICLE Open Access Anti-inflammatory activity of monogalactosyldiacylglycerol in human articular cartilage in vitro activation of an antiinflammatory cyclooxygenase-2 COX-2 pathway 1 f 2f 1 3 Valentina Ulivi Manuela Lenti Chiara Gentili Gabriele Marcolongo Ranieri Cancedda 1 and Fiorella Descalzi Cancedda1 4 Abstract Introduction The mono- and digalactosyldiacylglycerol MGDG and DGDG galactolipids have been purified from the thermophilic blue-green alga Phormidium sp. ETS-05 that colonizes the therapeutic thermal mud of Abano Terme and Montegrotto Terme Italy. Both compounds present a marked composition in polyunsaturated fatty acids mainly omega-3. The therapeutic thermal mud is applied mainly to osteoarthritic cartilage patients. In the present study the effect of MGDG treatment on proteins and factors expressed by human articular cartilage cells in culture and on pathways activated in inflammatory conditions was studied. Methods Primary cultures of human articular chondrocytes were used at cell passage number 1 P1 . Cells were treated in serum-free medium with inflammatory cytokines in the presence and in the absence of MGDG. Western blot was performed on collected medium and on cell layers. At least three different experiments were performed on primary cultures. The quantitation of the MGDG effect was performed by densitometric scanning of Western blots. p38 Mitogen Activated Protein Kinase p38 activation Nuclear Factor-kappaB NF-kB activation and Prostaglandin E2 PGE2 quantitation were performed by commercially available assays. Results are given as the mean values SD. All statistical analyses were performed using GraphPad software. The two-tailed Student s t -test was performed. Results We report that MGDG 1 represses the expression of interleukin-6 IL-6 and interleukin-8 IL-8 induced by interleukin-1alpha IL-1a or IL-1a tumor necrosis factor a TNFa
