Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Distinct host cell proteins incorporated by SIV replicating in CD4+ T Cells from natural disease resistant versus non-natural disease susceptible hosts. | Stephenson et al. Retrovirology 2010 7 107 http content 7 1 107 RETROVIROLOGY RESEARCH Open Access Distinct host cell proteins incorporated by SIV replicating in CD4 T Cells from natural disease resistant versus non-natural disease susceptible hosts Susan T Stephenson1 Pavel Bostik2 3 Byeongwoon Song4 Devi Rajan4 Samrath Bhimani1 Pavel Rehulka2 Ann E Mayne1 Aftab A Ansari1 Abstract Background Enveloped viruses including the simian immunodeficiency virus SIV replicating within host cells acquire host proteins upon egress from the host cells. A number of studies have catalogued such host proteins and a few have documented the potential positive and negative biological functions of such host proteins. The studies conducted herein utilized proteomic analysis to identify differences in the spectrum of host proteins acquired by a single source of SIV replicating within CD4 T cells from disease resistant sooty mangabeys and disease susceptible rhesus macaques. Results While a total of 202 host derived proteins were present in viral preparations from CD4 T cells from both species there were 4 host-derived proteins that consistently and uniquely associated with SIV replicating within CD4 T cells from rhesus macaques but not sooty mangabeys and similarly 28 host-derived proteins that uniquely associated with SIV replicating within CD4 T cells from sooty mangabeys but not rhesus macaques. Of interest was the finding that of the 4 proteins uniquely present in SIV preparations from rhesus macaques was a 26 S protease subunit 7 MSS1 that was shown to enhance HIV-1 tat mediated transactivation. Among the 28 proteins found in SIV preparations from sooty mangabeys included several molecules associated with immune function such as CD2 CD3e TLR4 TLR9 and TNFR and a bioactive form of IL-13. Conclusions The finding of 4 host proteins that are uniquely associated with SIV replicating within CD4 T cells from disease susceptible rhesus macaques and 28 host proteins .
