Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:"Recovery of fitness of a live attenuated simian immunodeficiency virus through compensation in both the coding and non-coding regions of the viral genome. | Retrovirology BioMed Central Research Recovery of fitness of a live attenuated simian immunodeficiency virus through compensation in both the coding and non-coding regions of the viral genome James B Whitney1 2 3 and Mark A Wainberg 1 2 Open Access Address McGill University AIDS Centre Lady Davis Institute-Jewish General Hospital Montreal Quebec H3T 1E2 Canada 2Department of Microbiology and Immunology McGill University Montreal Quebec H3A 2B4 Canada and 3Division of Viral Pathogenesis Beth Israel Deaconess Medical Center Harvard Medical School Boston MA 02115 USA Email James B Whitney - jwhitne2@ Mark AWainberg - Corresponding author Published 3 July 2007 Received 7 February 2007 Retrovirology 2007 4 44 doi 1742-4690-4-44 Accepted 3 July 2007 This article is available from http content 4 1 44 2007 Whitney and Wainberg licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract We have analyzed a SIV deletion mutant that was compromised both in viral replication and RNA packaging. Serial passage of this variant in two different T-cell lines resulted in compensatory reversion and the generation of independent groups of point mutations within each cell line. Within each group single point mutations were shown to contribute to increased viral infectivity and the rescue of wild-type replication kinetics. The complete recovery of viral fitness ultimately correlated with the restoration of viral RNA packaging. Consistent with the latter finding was the rescue of Pr55 Gag processing also restoring proper virus core morphology in mature virions. These seemingly independently arising groups of compensatory mutations were functionally interchangeable in regard to the
