Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: "Isolated HIV-1 core is active for reverse transcription. | Retrovirology BioMed Central Short report Isolated HIV-I core is active for reverse transcription David Warrilow1 Deborah Stenzel2 and David Harrich 1 Open Access Address 1Division of Immunology and Infectious Disease Queensland Institute of Medical Research Brisbane Queensland 4006 Australia and 2Analytical Electron Microscopy Facility Queensland University of Technology Gardens Point Campus Brisbane Queensland 4001 Australia Email David Warrilow - Deborah Stenzel - David Harrich - davidH@ Corresponding author Published 24 October 2007 Received 28 August 2007 Accepted 24 October 2007 Retrovirology 2007 4 77 doi 1742-4690-4-77 This article is available from http content 4 1 77 2007 Warrilow et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Whether purified HIV-I virion cores are capable of reverse transcription or require uncoating to be activated is currently controversial. To address this question we purified cores from a virus culture and tested for the ability to generate authentic reverse transcription products. A dense fraction approximately g ml prepared without detergent possibly derived from disrupted virions was found to naturally occur as a minor sub-fraction in our preparations. Core-like particles were identified in this active fraction by electron microscopy. We are the first to report the detection of authentic strong-stop first-strand transfer and full-length minus strand products in this core fraction without requirement for an uncoating activity. Findings Deoxyribonucleotides added directly to HIV-1 virions are incorporated into reverse transcription products 1-4 . This process which is reported to .
