Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Analysis of variation of amplitudes in cell cycle gene expression | Theoretical Biology and Medical Modelling BioMed Central Research Open Access Analysis of variation of amplitudes in cell cycle gene expression Delong Liu 1 2 Kevin W Gaido1 and Russ Wolfinger2 Address 1CIIT Ceters for Health Research 6 Davis Drive Research Triangle Park NC 27709 USA and 2The SAS Institute Inc. SAS Campus Drive Cary NC 27513 USA Email Delong Liu - dliu@ Kevin W Gaido - gaido@ Russ Wolfinger - Corresponding author Published II November 2005 Received 31 August 2005 Accepted 11 November 2005 Theoretical Biology and Medical Modelling 2005 2 46 doi 1742-4682-2-46 H This article is available from http content 2 1 46 2005 Liu et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Variation in gene expression among cells in a population is often considered as noise produced from gene transcription and post-transcription processes and experimental artifacts. Most studies on noise in gene expression have emphasized a few well-characterized genes and proteins. We investigated whether different cell-arresting methods have impacts on the maximum expression levels amplitudes of a cell cycle related gene. Results By introducing random noise modeled by a von Mises distribution to the phase angle in a sinusoidal model in a cell population we derived a relationship between amplitude and the distribution of noise in maximum transcription time phase . We applied our analysis to Whitfield s HeLa cell cycle data. Our analysis suggests that among 47 cell cycle related genes common to the 2nd experiment thymidine-thymidine method and the 4th experiment thymidine-nocodazole method i the amplitudes of CDC6 and PCNA which are expressed during G1 S phase
