Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Comment on and reply to "Analysis of variation of amplitudes in cell cycle gene expression" by Liu, Gaido and Wolfinger: On the analysis of gene expression during the normal, eukaryotic, cell cycle | Theoretical Biology and Medical Modelling Commentary BioMed Central Open Access Comment on and reply to Analysis of variation of amplitudes in cell cycle gene expression by Liu Gaido and Wolfinger On the analysis of gene expression during the normal eukaryotic cell cycle Stephen Cooper Address Department of Microbiology and Immunology University of Michigan Medical School Ann Arbor Michigan 48109-0620 USA Email Stephen Cooper - cooper@ Corresponding author Published 18 November 2005 Received 13 November 2005 Accepted 18 November 2005 Theoretical Biology and Medical Modelling 2005 2 47 doi 1742-4682-2-47 H This article is available from http content 2 1 47 2005 Cooper licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The paper of Liu Gaido and Wolfinger on gene expression during the division cycle of HeLa cells using the data of Whitfield et al. are discussed in order to see whether their analysis is related to gene expression during the division cycle. Results The results of Liu Gaido and Wolfinger demonstrate that different inhibition methods proposed to synchronize cells lead to different levels of gene expression. This result in and of itself should be taken as evidence that the original work of Whitfield et al. is flawed and should not be used to support the notion that the cells studied were synchronized or that the microarray analyses identify cell-cycle-regulated genes. Furthermore the DNA content evidence presented by Whitfield et al. supports the proposal that the cells described as synchronized are not synchronized. A comparison of the gene expression amplitudes from two different experiments indicates that the results are not reproducible. Conclusion It is .
