Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Global nucleosome occupancy in yeast. | Research Open Access Global nucleosome occupancy in yeast Bradley E Bernstein Chih Long Liu Emily L Humphrey Ethan O Perlstein and Stuart L Schreiber Addresses Department of Chemistry and Chemical Biology Bauer Center for Genomics Research and Howard Hughes Medical Institute Harvard University 12 Oxford Street Cambridge MA 02138 USA. Department of Pathology Brigham and Women s Hospital and Harvard Medical School Boston MA 02115 USA. Biological and Biomedical Sciences Division of Medical Sciences Harvard Medical School Boston MA 02115 USA. Department of Molecular and Cellular Biology Harvard University Cambridge MA 02138 USA. Correspondence Bradley E Bernstein. E-mail bbernstein@. Stuart L Schreiber. E-mail stuart_schreiber@ Published 20 August 2004 Genome Biology 2004 5 R62 The electronic version of this article is the complete one and can be found online at http 2004 5 9 R62 Received 16 May 2004 Revised 16 July 2004 Accepted 4 August 2004 2004 Bernstein et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Although eukaryotic genomes are generally thought to be entirely chromatin-associated the activated PHO5 promoter in yeast is largely devoid of nucleosomes. We systematically evaluated nucleosome occupancy in yeast promoters by immunoprecipitating nucleosomal DNA and quantifying enrichment by microarrays. Results Nucleosome depletion is observed in promoters that regulate active genes and or contain multiple evolutionarily conserved motifs that recruit transcription factors. The Rapl consensus was the only binding motif identified in a completely unbiased search of nucleosome-depleted promoters. Nucleosome depletion in the vicinity of Rapl consensus sites in .
