Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh Original cung cấp cho các bạn kiến thức về sinh học đề tài: Assignment of PCR markers to river buffalo chromosomes. | 71 Genet. Sei. Evol. 30 1998 71-78 Inra Elsevier Paris Note Assignment of PCR markers to river buffalo chromosomes Hanaa A. Orabya Soheir M. El Nahas H. Anna de Hondta Akmal El Ghorb Mohamed F. Abdel Samada a Department of Cell Biology National Research Centre Dokki Cairo Egypt b Department of Zoology Faculty of Science Cairo University Cairo Egypt Received 4 June 1997 accepted 25 September 1997 Abstract - In the process of developing a buffalo physical map using somatic cell hybrids and the cattle gene map as a template ten PCR primers designed for four coding genes F10 FSHB HBB and CYM and six DNA segments TGLA9 TGLA227 UWCA5 CSSM6 CSSM47 and RF131 were tested on a panel of 47 buffalo-hamster somatic cell hybrids. F10-TGLA9 FSHB-HBB and UWCA5-TGLA227 respectively were found to segregate together forming three syntenic groups. These three syntenic groups have also been reported in cattle where they have been assigned to chromosomes BTA 12 BTA 15 and BTA 18 respectively. Comparative mapping predicts the assignment of these syntenic groups to river buffalo chromosomes BBU 13 BBU 16 and BBU 18 respectively. Inra Elsevier Paris buffalo chromosome synteny PCR markers gene mapping Resume Assignation de marqueurs PCR aux chromosomes du buffle de riviere. Dans le but de développer la carte génétique physique du buffie en utilisant 1 hybridation cellulaire somatique et la carte génétique bovine comme reference dix amorces PCR correspondant à quatre genes codants Flo FSHB HBB et CYM et six segments d ADN TGLA9 TGLA227 UWCA5 CSSM6 CSSM47 et RF131 on été testés sur une série de 47 hybrides cellulaires somatiques entre bufHe et hamster. F10-TGLA9 FSHB-HBB et UWCA5-TGLA227 respectivement ont été trouvés ségréger ensemble pour former trois groupes synténiques. Ceux-ci ont été aussi observes chez les bovins où ils ont été assignés aux chromosomes BTA 12 BTA 15 et BTA 18 respectivement. Les cartes comparées permettent de prédire une assignation respective des trois groupes .
