Service de Conformation des Macromolécules Biologiques, Centre de Biologie Structurale et Bioinformatique, CP 263, Université Libre de Bruxelles, Bld du Triomphe, B-1050 Bruxelles, Belgium. †Institut Pasteur, Unité d'Expression des Gènes Eucaryotes, Institut Pasteur, rue du Docteur Roux, 75724 Paris Cedex 15, France. Correspondence: Shoshana J Wodak. E-mail: shosh@ reviews Published: 30 April 2004 Genome Biology 2004, 5:R33 The electronic version of this article is the complete one and can be found online at . | Research Open Access Transcriptional regulation of protein complexes in yeast Nicolas Simonis Jacques van Helden George N Cohen and Shoshana J Wodak Addresses Service de Conformation des Macromolécules Biologiques Centre de Biologie Structurale et Bioinformatique CP 263 Université Libre de Bruxelles Bld du Triomphe B-1050 Bruxelles Belgium. ỶInstitut Pasteur Unité d Expression des Gènes Eucaryotes Institut Pasteur rue du Docteur Roux 75724 Paris Cedex 15 France. Correspondence ShoshanaJWodak. E-mail shosh@ Published 30 April 2004 Genome Biology 2004 5 R33 The electronic version of this article is the complete one and can be found online at http 2004 5 5 R33 Received 26 November 2003 Revised 30 March 2004 Accepted 6 April 2004 2004 Simonis et al. licensee BioMed Central Ltd. This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract Background Multiprotein complexes play an essential role in many cellular processes. But our knowledge of the mechanism of their formation regulation and lifetimes is very limited. We investigated transcriptional regulation of protein complexes in yeast using two approaches. First known regulons manually curated or identified by genome-wide screens were mapped onto the components of multiprotein complexes. The complexes comprised manually curated ones and those characterized by high-throughput analyses. Second putative regulatory sequence motifs were identified in the upstream regions of the genes involved in individual complexes and regulons were predicted on the basis of these motifs. Results Only a very small fraction of the analyzed complexes 5-6 have subsets of their components mapping onto known regulons. Likewise regulatory motifs are detected in only about 8-15 of the complexes and in those about half of the components are on average part of predicted
