Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Reduction in antioxidant enzyme expression and sustained inflammation enhance tissue damage in the subacute phase of spinal cord contusive injury | Wang et al. Journal of Biomedical Science 2011 18 13 http content 18 1 13 The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access Reduction in antioxidant enzyme expression and sustained inflammation enhance tissue damage in the subacute phase of spinal cord contusive injury 12 2 3 3 24 Chih-Yen Wang Jen-Kun Chen Yi-Ting Wu May-Jywan Tsai Song-Kun Shyue Chung-Shi Yang Shun-Fen Tzeng1 Abstract Background Traumatic spinal cord injury SCI forms a disadvantageous microenvironment for tissue repair at the lesion site. To consider an appropriate time window for giving a promising therapeutic treatment for subacute and chronic SCI global changes of proteins in the injured center at the longer survival time points after SCI remains to be elucidated. Methods Through two-dimensional electrophoresis 2DE -based proteome analysis and western blotting we examined the differential expression of the soluble proteins isolated from the lesion center LC at day 1 acute and day 14 subacute after a severe contusive injury to the thoracic spinal cord at segment 10. In situ apoptotic analysis was used to examine cell apoptosis in injured spinal cord after adenoviral gene transfer of antioxidant enzymes. In addition administration of chondroitinase ABC chABC was performed to analyze hindlimb locomotor recovery in rats with SCI using Basso Beattie and Bresnahan BBB locomotor rating scale. Results Our results showed a decline in catalase CAT and Mn-superoxide dismutase MnSOD found at day 14 after SCI. Accordingly gene transfer of SOD was introduced in the injured spinal cord and found to attenuate cell apoptosis. Galectin-3 p-actin actin regulatory protein CAPG and F-actin-capping protein subunit p CAPZB at day 14 were increased when compared to that detected at day 1 after SCI or in sham-operated control. Indeed the accumulation of p-actin immune cells was observed .
