Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Catechin hydrate suppresses MCF-7 proliferation through TP53/Caspase-mediated apoptosis | Journal of Experimental Clinical Cancer Research Alshatwi Journal of Experimental Clinical Cancer Research 2010 29 167 http content 29 1 167 RESEARCH Open Access Catechin hydrate suppresses MCF-7 proliferation through TP53 Caspase-mediated apoptosis Ali A Alshatwi Abstract Catechin hydrate CH a strong antioxidant that scavenges radicals is a phenolic compound that is extracted from plants and is present in natural food and drinks such as green tea and red wine. CH possesses anticancer potential. The mechanism of action of many anticancer drugs is based on their ability to induce apoptosis. In this study I sought to characterize the downstream apoptotic genes targeted by CH in MCF-7 human breast cancer cells. CH effectively kills MCF-7 cells through induction of apoptosis. Apoptosis was confirmed by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling TUNEL and real-time PCR assays. Cells were exposed to 150 pg ml CH and 300 pg mL CH for 24 hours which resulted in and apoptotic cells respectively. Moreover a 48-hour exposure to 150 pg ml CH and 300 pg ml CH resulted in and apoptotic cells respectively. Interestingly after 72 hours of exposure to both concentrations of CH almost 100 of cells lost their integrity. These results were further confirmed by the increased expression of caspase-3 -8 and -9 and TP53 in a time-dependent and dose-dependent manner as determined by real-time quantitative PCR. In summary the induction of apoptosis by CH is affected by its ability to increase the expression of pro-apoptotic genes such as caspase-3 -8 and -9 and TP53. Introduction Catechin compounds including - - epigallocatechin-3-gallate EGCG - - epigallocatechin EGC epicatechin-3-gallate ECG and p catechin 1 have been shown to exhibit cytostatic properties in many tumor models 2 3 . In addition the growth of new blood vessels required for tumor growth has been prevented by green tea 4 . In Asian countries a number of .
