Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Enhancive effects of Lewis y antigen on CD44-mediated adhesion and spreading of human ovarian cancer cell line RMG-I | Gao et al. Journal of Experimental Clinical Cancer Research 2011 30 15 http content 30 1 15 Journal of Experimental Clinical Cancer Research RESEARCH Open Access Enhancive effects of Lewis y antigen on CD44-mediated adhesion and spreading of human ovarian cancer cell line RMG-I 1 11 1 1 1 1 1 Lili Gao Limei Yan Bei Lin Jian Gao Xiuyun Liang Yanyan Wang Juanjuan Liu Shulan Zhang Iwamori Masao2 Abstract Background This study aimed to investigate the molecular structural relationship between cell adhesive molecule CD44 and Lewis y antigen and determine the effects of Lewis y antigen on CD44-mediated adhesion and spreading of ovarian cancer cell line RMG-I and the Lewis y antigen-overexpressed cell line RMG-I-H. Methods The expression of CD44 in RMG-I and RMG-I-H cells before and after treatment of Lewis y monoclonal antibody was detected by immunocytochemistry the expression of Lewis y antigen and CD44 was detected by Western Blot. The structural relationship between Lewis y antigen and CD44 was determined by immunoprecipitation and confocal laser scanning microscopy. The adhesion and spreading of RMG-I and RMG-I-H cells on hyaluronic acid HA were observed. The expression of CD44 mRNA in RMG-I and RMG-I-H cells was detected by real-time RT-PCR. Results Immunocytochemistry revealed that the expression of CD44 was significantly higher in RMG-I-H cells than in RMG-I cells P and its expression in both cell lines was significantly decreased after treatment of Lewis y monoclonal antibody both P . Western Blot confirmed that the content of CD44 in RMG-I-H cells was times of that in RMG-I cells. The co-location of Lewis y antigen and CD44 was confirmed by coimmunoprecipitation. The co-expression of CD44 and Lewis y antigen in RMG-I-H cells was times of that in RMG-I cells. The adhesion and spreading of RMG-I-H cells on HA were significantly enhanced as compared to those of RMG-I cells P and this enhancement was inhibited by Lewis y .
