Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Potential prognostic marker ubiquitin carboxylterminal hydrolase-L1 does not predict patient survival in non-small cell lung carcinoma | Orr et al. Journal of Experimental Clinical Cancer Research 2011 30 79 http content 30 1 79 Journal of Experimental Clinical Cancer Research RESEARCH Open Access Potential prognostic marker ubiquitin carboxyl-terminal hydrolase-LI does not predict patient survival in non-small cell lung carcinoma Katy S Orr Zhanzhong Shi W Mark Brown Kathleen A O Hagan Terence R Lappin Perry Maxwell and Melanie J Percy Abstract Background Ubiquitin Carboxyl-Terminal Hydrolase-L1 UCH-L1 is a deubiquitinating enzyme that is highly expressed throughout the central and peripheral nervous system and in cells of the diffuse neuroendocrine system. Aberrant function of UCH-L1 has been associated with neurological disorders such as Parkinson s disease and Alzheimer s disease. Moreover UCH-L1 exhibits a variable expression pattern in cancer acting either as a tumour suppressor or promoter depending on the type of cancer. In non-small cell lung carcinoma primary tumour samples UCH-L1 is highly expressed and is associated with an advanced tumour stage. This suggests UCH-L1 may be involved in oncogenic transformation and tumour invasion in NSCLC. However the functional significance of UCH-L1 in the progression of NSCLC is unclear. The aim of this study was to investigate the role of UCH-L1 using NSCLC cell line models and to determine if it is clinically relevant as a prognostic marker for advanced stage disease. Methods UCH-L1 expression in NSCLC cell lines H838 and H157 was modulated by siRNA-knockdown and the phenotypic changes were assessed by flow cytometry haematoxylin eosin H E staining and poly ADP-ribose polymerase PARP cleavage. Metastatic potential was measured by the presence of phosphorylated myosin light chain MLC2 . Tumour microarrays were examined immunohistochemically for UCH-L1 expression. Kaplan-Meier curves were generated using UCH-L1 expression levels and patient survival data extracted from Gene Expression Omnibus data files. Results Expression of UCH-L1 was
