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Handbook of Microbiological Media, Fourth Edition part 131

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Handbook of Microbiological Media, Fourth Edition part 131 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Nitrobacter Medium B 1295 Solution E.0.5mL Solution F.0.2mL Solution A Composition per 100.0mL CaCl2.2.0g Preparation of Solution A Add CaCl2 to distilled deionized water and bring volume to 100.0mL. Mix thoroughly. Solution B Composition per 100.0mL MgSO4-7H2O.20.0g Preparation of Solution B Add MgSO4-7H2O to distilled deion-ized water and bring volume to 100.0mL. Mix thoroughly. Solution C Composition per 100.0mL Chelated iron Sequestrene .0.1g Preparation of Solution C Add chelated iron to distilled deion-ized water and bring volume to 100.0mL. Mix thoroughly. Solution D Composition per liter MnCl24H2O.0.2g Na2MoO4-2H2O.0.1g ZnSO4-7H2O.0.1g CuSO4-5H2O.0.02g CoCl26H2O.2.0mg Preparation of Solution D Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Solution E Composition per 100.0mL NaNO2.41.4g Preparation of Solution E Add NaNO2 to distilled deionized water and bring volume to 100.0mL. Mix thoroughly. Solution F Composition per 100.0mL K2HPO4.1.74g Preparation of Solution F Add K2HPO4 to distilled deionized water and bring volume to 100.0mL. Mix thoroughly. Preparation of Medium Add the appropriate volumes of solutions A-F to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation and maintenance of Nitrobacter species and Nitrobacter winogradskyi. Nitrobacter Medium 204 Composition per liter Seawater.700.0mL Solution C.1.0mL Solution A.0.5mL Solution B.0.5mL Solution D.0.5mL Solution E.0.5mL Solution F.0.2mL Solution A Composition per 100.0mL CaCl2.2.0g Preparation of Solution A Add CaCl2 to distilled deionized water and bring volume to 100.0mL. Mix thoroughly. Solution B Composition per 100.0mL MgSO4-7H2O.20.0g Preparation of Solution B Add MgSO4-7H2O to distilled deionized water and bring volume to 100.0mL. Mix thoroughly. Solution C Composition per 100.0mL Chelated iron Sequestrene .0.1g Preparation of .