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Báo cáo y học: "Correction: Catabolic stress induces expression of hypoxiaα inducible factor (HIF)-1α in articular chondrocytes: involvement α of HIF-1α in the pathogenesis of osteoarthritis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Correction: Catabolic stress induces expression of hypoxiaα inducible factor (HIF)-1α in articular chondrocytes: involvement α of HIF-1α in the pathogenesis of osteoarthritis. | Available online http arthritis-research.eom content 7 5 225 Correction Correction Catabolic stress induces expression of hypoxiainducible factor HIF -1a in articular chondrocytes involvement of HIF-1a in the pathogenesis of osteoarthritis Kazuo Yudoh Hiroshi Nakamura Kayo Masuko-Hongo Tomohiro Kato and Kusuki Nishioka Department of Bioregulation Institute of Medical Science St. Marianna University School of Medicine Kawasaki Japan Corresponding author Kazuo Yudoh yudo@marianna-u.ac.jp Published 31 August 2005 This article is online at http arthritis-research.com content 7 5 225 2005 BioMed Central Ltd Arthritis Research Therapy 2005 7 225 DOI 10.1186 ar1822 In our recent article 1 we did not identify three paragraphs in the Material and Methods section that were reproduced from an article by Pfander et al. 2 . This omission has been corrected by the addition of a reference to the Pfander et al. paper as follows Measurement of lactic acid in cultured chondrocytes To examine the role of HIF-1a in the glycosis in human OA chondrocytes the level of lactic acid was measured in culture chondrocytes by the method reported by Pfander et al. 21 . Supernatants from chondrocyte cultures were collected after 24 hours under normoxic or hypoxic conditions. Lactic acid was determined by a colorimetric assay Sigma at 540 nm in accordance with the manufacturer s instructions. Lactic acid levels were normalized to total protein content as measured by the Bradford assay Bio-Rad Hercules CA USA ATP levels in cultured chondrocytes To study the role of HIF-1a in energy generation in human OA chondrocytes the ATP level was analyzed in cultured chondrocytes by the method reported by Pfander et al. 21 . Chondrocytes were collected after a 24-hour incubation under normoxic or hypoxic conditions. The ATP Bioluminescence assay kit CLS II Roche Heidelberg Germany was used. The assay is based on the light-emitting oxidation of luciferin by luciferase in the presence of extremely low levels of